Flow cytometer performance: Fluorochrome dependent sensitivity and instrument configuration
نویسندگان
چکیده
منابع مشابه
Characterization of flow cytometer instrument sensitivity.
Fluorescence sensitivity, measured in terms of resolution, allows the researcher to more accurately answer the question of how dim a cell can be and still be resolvable from another population. This measure focuses on the width, i.e., the standard deviation, of the population distributions and not on the location, i.e., the mean intensity, of populations on a histogram scale relative to the bac...
متن کاملAutomated Quadratic Characterization of Flow Cytometer Instrument Sensitivity
Flow cytometer sensitivity has been well defined in the community and relates to two functions: (1) How well a dim staining population is resolved from an unstained population; (2) How well various dim staining populations can be distinguished from each other. The most common terms for characterizing flow cytometer sensitivity are known as Q (detector efficiency) and B (background light level),...
متن کاملflowQB – Automated Quadratic Characterization of Flow Cytometer Instrument Sensitivity*
This package provides methods to calculate flow cytometer’s detection efficiency (Q) and background illumination (B) by analyzing LED pulses and multi-level bead sets. The method improves on previous formulations Wood (1998); Hoffman and Wood (2007); Chase and Hoffman (1998) by fitting a full quadratic model with appropriate weighting, and by providing standard errors and peak residuals as well...
متن کاملAutomated Quadratic Characterization of Flow Cytometer Instrument Sensitivity
Flow cytometer sensitivity has been well defined in the community and relates to two functions: (1) How well a dim staining population is resolved from an unstained population; (2) How well various dim staining populations can be distinguished from each other. The most common terms for characterizing flow cytometer sensitivity are known as Q (detector efficiency) and B (background light level),...
متن کاملDevelopment of a diffraction imaging flow cytometer.
Diffraction images record angle-resolved distribution of scattered light from a particle excited by coherent light and can correlate highly with the 3D morphology of a particle. We present a jet-in-fluid design of flow chamber for acquisition of clear diffraction images in a laminar flow. Diffraction images of polystyrene spheres of different diameters were acquired and found to correlate highl...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1995
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990220412